CD25 / PE / 280406
CD25 / PE / 280406
Product Details
| Supplier | R&D Systems | |
|---|---|---|
| Catalog #: | FAB2438P-100 (View supplier product page) | |
| Size | 100 Tests | |
| Price | $249.00 | |
| Antigen | CD25 | |
| Clone | 280406 | |
| Host | Rat | |
| Isotype | IgG2a | |
| Conjugate | PE | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | Mouse CD25/IL-2R alpha PE-conjugated Antibody |
About CD25 and PE
| CD25 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| PE | Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts. |
Images
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Detection of CD25/IL-2 R alpha in CD4+ Mouse T Cells by Flow Cytometry. CD4+ mouse T cells treated with anti-mouse CD3 and anti-mouse CD28 for 24 hours were stained with Rat Anti-Mouse CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) and Rat Anti-Mouse CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB554F). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins. |
Citations
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PE Excitation and Emission Spectra
$249.00
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