Lyve-1 / FITC / REAL1161

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-128-001 (View supplier product page)
Size 200 μl
Price $400.00
Antigen Lyve-1
Clone REAL1161
Host cell line
Isotype IgG
Conjugate FITC
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IHC, IF
Description Clone REAL1161 is an antibody fragment derived from the full LYVE-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL1161 recognizes the human LYVE-1 protein, also known as lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE1, XLKD1 or CRSBP-1. LYVE-1 is a type I integral membrane glycoprotein, which is a lymph-specific receptor for hyaluronan and homologous to CD44. LYVE-1 is mainly expressed in endothelial cells lining lymphatic vessels, but also in normal hepatic blood sinusoidal endothelial cells. LYVE-1 is an indicator for human hepatocellular carcinoma. It is involved in lymphatic hyaluronan transport, in tumor metastasis and autocrine regulation of cell growth. Lyve-1 is expressed in liver cancer. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675). |
About Lyve-1 and FITC
Lyve-1 This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra