CD73 / FITC / REAL1172

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-129-891 (View supplier product page)
Size 200 μl
Price $415.00
Antigen CD73
Clone REAL1172
Host cell line
Isotype IgG
Conjugate FITC
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IHC, IF
Description Clone REAL1172 is an antibody fragment derived from the full CD73 (NT5E) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL1172 recognizes the human CD73 (NT5E) antigen, which is a 70 kDa membrane-bound enzyme that catalyzes the conversion of AMP to bioactive adenosine at neutral pH. This glycoprotein is also called ecto-5’-nucleotidase. CD73 is present on subsets of T and B lymphozytes, follicular dendritic cells, endothelial cells, and marrow stromal cells (MSCs). CD73 (NT5E) acts as an inhibitory immune checkpoint molecule, expressed on cancer cells. It has been associated with poor survival and recurrence in patients with breast and ovarian cancer. In addition to the enzymatic role of CD73, it is an adhesion molecule. It mediates the interaction between endothelial cells and lymphocytes and takes a role in cell migration of normal and neoplastic cells. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675). |
About CD73 and FITC
CD73 The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra