CD354 / APC-Vio770 / REA213

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-130-020 (View supplier product page)
Size 100 tests in 200 µL
Price $350.00
Antigen CD354
Clone REA213
Host Human
Isotype IgG1
Conjugate APC-Vio770
Target Species Human
Applications FC
Description Clone REA213 recognizes the human CD354 (TREM-1), a 30 kDa member of the triggering receptors expressed on myeloid cells (TREM) family of cell surface receptors. TREM receptors are immunoglobulin 'superfamily' members and contain a single variable-type immunoglobulin domain. Human TREM cluster is located on chromosome 6p21. Expression of TREM-1 is found on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, and macrophages. TREM-1 signals via ITAM-containing adaptor DAP12 and serves as an amplifier of cell activation in response to triggers such as LPS. TREM-1 activated cellular signalling molecules and pathways lead to generation of pro-inflammatory response such as expression and secretion of chemokines and cytokines including MCP-1, MIP-1a, interleukin 6 (IL-6), IL-8, and TNF. TREM-1 acts in a concerted manner with other signalling receptors such as TLRs and Nod-like receptors, leading to an amplification of the initial response. Expression of TREM-1 itself is upregulated in response to presence of LPS, microbial products, and cytokines released during activation response. A soluble form of TREM-1 (sTREM-1) is also detected in fluids from patients with infections. | Additional information: Clone REA213 displays negligible binding to Fc receptors. |
About CD354 and APC-Vio770
CD354 This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
APC-Vio770 APC-Vio®770 from Miltenyi is a tandem fluorophore with APC as the donor and has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), and APC-Fire™ 750 (BioLegend). The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
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APC-Vio770 Excitation and Emission Spectra