CD28 / Vio Bright V423 / REA612

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-127-223 (View supplier product page)
Size 30 tests in 60 µL
Price $170.00
Antigen CD28
Clone REA612
Host Human
Isotype IgG1
Conjugate Vio Bright V423
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
Description Clone REA612 recognizes the human CD28 antigen, a type I transmembrane protein, which is highly expressed on CD3+ thymocytes, most peripheral thymocytes, and plasma cells but not in less mature B cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production in vitro and in vivo. In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production. | Additional information: Clone REA612 displays negligible binding to Fc receptors.
About CD28 and Vio Bright V423
CD28 The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Vio Bright V423 Vio® Bright V423 is a very bright Violet emitting synthetic fluorophore used in flow cytometry. The excitation and emission peaks are 399 nm and 420 nm, respectively, which means that this dye is optimally excited by the 405 nm Violet laser while the emission light is collected by a 450/50 bandpass filter.
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Vio Bright V423 Excitation and Emission Spectra