CD43 / Alexa Fluor 647 / 84-3C1

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP3-12075AF647 (View supplier product page)
Size 100 μl
Price $519.00
Antigen CD43
Clone 84-3C1
Host Rabbit
Isotype IgG κ
Conjugate Alexa Fluor 647
Target Species Human
Applications WB, FC, IHC, ICC/IF
Description CD43/Sialophorin Antibody (84-3C1) [Alexa Fluor® 647]
About CD43 and Alexa Fluor 647
CD43 This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra