Akt1 Monoclonal / APC / REA134

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-099-475 (View supplier product page)
Size 30 tests in 300 µL
Price $145.00
Antigen Akt1
Clone REA134
Host Human
Isotype IgG1
Conjugate APC
Target Species Human
Applications FC
Description Clone REA134 recognizes AKT1, which is also known as protein kinase Bα. AKT1 is a serine/threonine protein kinase, belonging to the AKT family of kinases and like each AKT family member, contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a carboxyl-terminal regulatory domain with a hydrophobic motif (HM). Activation of AKT1 is achieved via phosphorylation at multiple sites, which take place in response to engagement of receptors such as platelet derived growth factor receptor (PDGF-R). Activated AKT1 further phosphorylates and alters the activity of several downstream substrates allowing AKT1 to play a vital role in various biological processes such as cell growth, survival, migration, and proliferation. | Additional information: Clone REA134 displays negligible binding to Fc receptors. |
About Akt1 and APC
Akt1 This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]
APC Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
Citations
PMID 20042722
PMID 22647600
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APC Excitation and Emission Spectra