FPR1 Monoclonal / FITC / REA169

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-100-817 (View supplier product page)
Size 30 tests in 300 µL
Price $95.00
Antigen FPR1
Clone REA169
Host Human
Isotype IgG1
Conjugate FITC
Target Species Human
Applications FC, Mass Cytometry
Description Clone REA169 recognizes N-formyl-L-methionyl-L-leucyl-phenylalanine receptor (fMLP receptor), which is a seven transmembrane G-protein coupled receptor. fMLP receptor binds formylated peptides such as fMLP, which originate from degraded bacterial or mitochondrial proteins and mediate inflammatory reactions, activation of neutrophils and trafficking of leukocytes to the site of inflammation. Expression of fMLP receptor is mainly found on leukocytes, neutrophils and monocytes. | Additional Information: Clone REA169 displays negligible binding to Fc receptors. |
About FPR1 and FITC
FPR1 This gene encodes a G protein-coupled receptor of mammalian phagocytic cells that is a member of the G-protein coupled receptor 1 family. The protein mediates the response of phagocytic cells to invasion of the host by microorganisms and is important in host defense and inflammation.[provided by RefSeq, Jul 2010]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Citations
PMID 14556648
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FITC Excitation and Emission Spectra