MSR1 Monoclonal / FITC / REA148

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-102-251 (View supplier product page)
Size 30 μg in 1 ml
Price $60.00
Antigen MSR1
Clone REA148
Host Human
Isotype IgG1
Conjugate FITC
Target Species Mouse
Applications FC
Description Clone REA148 recognizes mouse CD204, also known as MSR 1 or SRA (murine scavenger receptor class A type I and II). It supports the recognition and ingestion of apoptotic cells and in particular it plays a supporting role in apoptotic cell clearance in embryogenesis. CD204 is mainly expressed on tissue macrophages. | Additional information: Clone REA148 displays negligible binding to Fc receptors. |
About MSR1 and FITC
MSR1 This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Citations
PMID 15580571
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FITC Excitation and Emission Spectra