CD158f Monoclonal / PE-Vio770 / UP-R1

Product Details
Supplier Miltenyi Biotec
Catalog #: 130-105-582 (View supplier product page)
Size 30 tests in 300 µL
Price $165.00
Antigen CD158f
Clone UP-R1
Host Mouse
Isotype IgG1,κappa
Conjugate PE-Vio770
Target Species Human
Applications FC
Description Clone UP-R1 recognizes CD158f (KIR2DL5), a member of the killer immunoglobulin-like receptor (KIR) family which recognizes subsets of HLA alleles. Expression is found mainly on CD56dimCD16+ natural killer (NK) cells but also on a subset of CD8+ T cells. KIRs are monomeric receptors possessing high allelic polymorphism, with either two or three Ig-like extracellular domains.
About CD158f and PE-Vio770
CD158f Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]
PE-Vio770 PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
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PE-Vio770 Excitation and Emission Spectra