CD8a / PE-Vio770 / BW135/80

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-098-060 (View supplier product page)
Size 30 Tests
Price $140.00
Antigen CD8a
Clone BW135/80
Host Mouse
Isotype IgG2a κ
Conjugate PE-Vio770
Target Species Human, Non-Human Primate
Applications FC
Description Detection of human cytotoxic T cells, detection of CD8+ non-human primate cells
About CD8a and PE-Vio770
CD8a The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
PE-Vio770 PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
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PE-Vio770 Excitation and Emission Spectra