CD38 Monoclonal / PerCP-Vio700 / 90.4

Citations: (4)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-103-788 (View supplier product page)
Size 30 μg in 1 ml
Price $170.00
Antigen CD38
Clone 90.4
Host Rat
Isotype IgG2a κ
Conjugate PerCP-Vio700
Target Species Mouse
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone 90.4 reacts with CD38, a 42 kDa transmembrane glycoprotein. CD38 is expressed on B cells, NK cells and a subset of thymocytes and peripheral T cells. CD38 expression is downregulated on mouse germinal center B cells and mouse mature plasma cells. The CD38 molecule, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and plays a role in lymphocyte activation.
About CD38 and PerCP-Vio700
CD38 The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
PerCP-Vio700 PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Citations
PMID 7734417
PMID 8235624
PMID 9013949
PMID 9694721
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PerCP-Vio700 Excitation and Emission Spectra