Perforin Monoclonal / FITC / delta G9

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-096-668 (View supplier product page)
Size 100 Tests in 1 ml
Price $265.00
Antigen Perforin
Clone delta G9
Host Mouse
Isotype IgG2b κ
Conjugate FITC
Target Species Human
Applications FC
Description The clone delta G9 reacts with human perforin, a 70 kDa cytolytic protein, which is expressed by cytotoxic T lymphocytes and natural killer (NK) cells, and which is stored in cytoplasmic granules. Upon cell activation perforin is released and functions as one of the major effector molecule to mediate killing of target cells.
About Perforin and FITC
Perforin This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra