CD2 Monoclonal / PerCP-Vio700 / LT2

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-105-166 (View supplier product page)
Size 30 tests in 300 µL
Price $155.00
Antigen CD2
Clone LT2
Host Mouse
Isotype IgG2b κ
Conjugate PerCP-Vio700
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Functional
Description The CD2 antibody recognizes the human CD2 antigen, a 50 kDa single-chain transmembrane glycoprotein also known as LFA-2 or receptor for sheep erythrocytes. CD2 belongs to the Ig superfamily and is involved in cell signaling and lymphocyte adhesion. The CD2 antibody reacts with 80–90% of peripheral blood lymphocytes and more than 95% of thymocytes. The antibody recognizes all T cells and a subset of natural killer (NK) cells.
About CD2 and PerCP-Vio700
CD2 The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene. [provided by RefSeq, Jun 2016]
PerCP-Vio700 PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Citations
PMID 9379483
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PerCP-Vio700 Excitation and Emission Spectra