FluoroFinder

	Supplier	US Biological
	Catalog #:	131043-FITC-100UL (View supplier product page)
	Size	100 μl
	Price	$681.00
	Antigen	PDE10A
	Clone	40
	Host	Mouse
	Isotype	IgG1 κ
	Conjugate	FITC
	Target Species	Human
	Applications	ELISA
	Description	PDE10A (cAMP and cAMP-inhibited cGMP 3',5'-cyclic Phosphodiesterase 10A, FLJ11894, FLJ25677, HSPDE10A) (FITC) Mab 4E1

	PDE10A	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

	Additional Sources

PDE10A / FITC / 40