IFN-gamma / Brilliant Violet 785 / 4S.B3

Citations: (18)
Product Details
Supplier BioLegend
Catalog #: 502542 (View supplier product page)
Size 100T
Price $446.00
Antigen IFN-gamma
Clone 4S.B3
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Violet 785
Target Species Human
Applications ICS
Description Brilliant Violet 785 anti-human IFN-gamma
About IFN-gamma and Brilliant Violet 785
IFN-gamma This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
Brilliant Violet 785 Brilliant™ Violet 785 (BV785) is a far-red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter. BV785 has an excitation peak at 405 nm and an emission peak at 785 nm. BV785 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV785 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  IFN-gamma Brilliant Violet 785 BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:4S.B3

PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 FITC, and then intracellularly stained with IFN-γ (clone 4S.B3) Brilliant Violet 785™.

Citations
PMID 17641010
Additional
Sources
Reviews & Ratings
Brilliant Violet 785 Excitation and Emission Spectra