CD23 / Brilliant Violet 421 / B3B4
CD23 / Brilliant Violet 421 / B3B4
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 101621 (View supplier product page) | |
| Size | 50 μg | |
| Price | $216.00 | |
| Antigen | CD23 | |
| Clone | B3B4 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | Brilliant Violet 421 anti-mouse CD23 |
About CD23 and Brilliant Violet 421
| CD23 | The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] | |
|---|---|---|
| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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C57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). |
Citations
| PMID 21454413 | ||
|---|---|---|
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$216.00
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