CD23 / PerCP-Cy5.5 / B3B4

Citations: (8)
Product Details
Supplier BioLegend
Catalog #: 101618 (View supplier product page)
Size 100 μg
Price $253.00
Antigen CD23
Clone B3B4
Host Rat
Isotype IgG2a κ
Conjugate PerCP-Cy5.5
Target Species Mouse
Applications FC
Description PerCP/Cyanine5.5 anti-mouse CD23
About CD23 and PerCP-Cy5.5
CD23 The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  CD23 PerCP-Cy5.5 BioLegend antibody anti target:Mouse host:Rat isotype:IgG2a κ clone:B3B4

C57BL/6 mouse splenocytes were stained with CD45R/B220 APC and CD23 (Clone B3B4) PerCP/Cyanine5.5 (left), or rat IgG2a, κ PerCP/Cyanine5.5 (right).

Citations
PMID 21454413
Additional
Sources
Reviews & Ratings
PerCP-Cy5.5 Excitation and Emission Spectra