CD235a / PE-Cy7 / HI264

Citations: (11)
Product Details
Supplier BioLegend
Catalog #: 349112 (View supplier product page)
Size 100T
Price $358.00
Antigen CD235a
Clone HI264
Host Mouse
Isotype IgG2a κ
Conjugate PE-Cy7
Target Species Human
Applications FC
Description PE/Cyanine7 anti-human CD235a (Glycophorin A)
About CD235a and PE-Cy7
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
PE-Cy7 PE-Cyanine®7 (PE-Cy7, RPE-Cy7) is a far red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy7. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy7, which emits light that can be captured with a 780/60 nm bandpass filter. PE-CY7 has an excitation peak at 565 nm and an emission peak at 778 nm, and is a suitable alternative to PE-Vio®770 (Miltenyi) and cFluor BYG781 (Cytek).
Images
  CD235a PE-Cy7 BioLegend antibody anti target:Human host:Mouse isotype:IgG2a κ clone:HI264

Human red blood cells were stained with anti-human CD235a (clone HI264) PE/Cy7 (filled histogram) or mouse IgG2a, κ PE/Cy7 isotype control (open histogram).

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PE-Cy7 Excitation and Emission Spectra