FluoroFinder

	IL-9	The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
	PerCP-Cy5.5	PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.

		Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cy5.5 (top) or rat IgG1, κ PerCP/Cy5.5 isotype control (bottom).