CD235a / PerCP-Cy5.5 / HI264

Citations: (5)
Product Details
Supplier BioLegend
Catalog #: 349109 (View supplier product page)
Size 25T
Price $176.00
Antigen CD235a
Clone HI264
Host Mouse
Isotype IgG2a κ
Conjugate PerCP-Cy5.5
Target Species Human
Applications FC
Description PerCP/Cyanine5.5 anti-human CD235a (Glycophorin A)
About CD235a and PerCP-Cy5.5
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  CD235a PerCP-Cy5.5 BioLegend antibody anti target:Human host:Mouse isotype:IgG2a κ clone:HI264

Human red blood cells were stained with anti-human CD235a (clone HI264) PerCP/Cy5.5 (filled histogram) or mouse IgG2a, κ PerCP/Cy5.5 isotype control (open histogram).

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PerCP-Cy5.5 Excitation and Emission Spectra