Ki-67 / Brilliant Violet 421 / 16A8
Ki-67 / Brilliant Violet 421 / 16A8
Citations:
(46)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 652411 (View supplier product page) | |
| Size | 50 μg | |
| Price | $286.00 | |
| Antigen | Ki-67 | |
| Clone | 16A8 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | Brilliant Violet 421 | |
| Target Species | Mouse | |
| Applications | ICS | |
| Description | Brilliant Violet 421 anti-mouse Ki-67 |
About Ki-67 and Brilliant Violet 421
| Ki-67 | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] | |
|---|---|---|
| Brilliant Violet 421 | Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). |
Citations
| PMID 25460664 | ||
|---|---|---|
| PMID 25675507 | ||
| PMID 25911761 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 421 Excitation and Emission Spectra
$286.00
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