FluoroFinder

	Supplier	BioLegend
	Catalog #:	338521 (View supplier product page)
	Size	25T
	Price	$176.00
	Antigen	CD23
	Clone	EBVCS-5
	Host	Mouse
	Isotype	IgG1 κ
	Conjugate	Brilliant Violet 421
	Target Species	Human
	Applications	FC
	Description	Brilliant Violet 421 anti-human CD23

	CD23	The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
	Brilliant Violet 421	Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue. Other similar fluorophores include SuperNova V428 (Beckman Coulter), StarBright Violet 440 (Bio-Rad) and SuperBright 436 (Thermo Fisher). BV421 is very bright and is most commonly used for flow cytometry. BV421 is very photo- and fixative stable. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dyes from Beckman Coulter and StarBright dyes from Bio-Rad.

		Human peripheral blood lymphocytes were stained with CD19 APC and CD23 (clone EBVCS-5) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).