p53 / PE / DO-7

Citations: (10)
Product Details
Supplier BioLegend
Catalog #: 645806 (View supplier product page)
Size 100T
Price $323.00
Antigen p53
Clone DO-7
Host Mouse
Isotype IgG2b κ
Conjugate PE
Target Species Human
Applications ICS
Description PE anti-p53
About p53 and PE
p53 This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Images
  p53 PE BioLegend antibody anti target:Human host:Mouse isotype:IgG2b κ clone:DO-7

A431 human breast cancer cell line was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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PE Excitation and Emission Spectra