FluoroFinder

	Supplier	BioLegend
	Catalog #:	145513 (View supplier product page)
	Size	125UL
	Price	$215.00
	Antigen	CD185
	Clone	L138D7
	Host	Rat
	Isotype	IgG2b κ
	Conjugate	Brilliant Violet 605
	Target Species	Mouse
	Applications	FC
	Description	Brilliant Violet 605 anti-mouse CD185 (CXCR5)

	CD185	This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
	Brilliant Violet 605	Brilliant™ Violet 605 (BV605) is an orange/red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 610/20 bandpass filter. BV605 has an excitation peak at 405 nm and an and emission peak at 603 nm. BV605 is most commonly used in flow cytometry and is spectrally similar to SuperBright 600 (Thermo Fisher), SuperNova v605 (Beckman Coulter) and StarBright Violet 610 (Bio-Rad). BV605 is considered to be relatively bright and should be used on antigens of moderate to low abudance. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.

		C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) Brilliant Violet 605™ (top) or rat IgG2b, κ Brilliant Violet 605™ isotype control (bottom).