CD19 / Brilliant Violet 510 / HIB19
CD19 / Brilliant Violet 510 / HIB19
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 302241 (View supplier product page) | |
| Size | 25T | |
| Price | $182.00 | |
| Antigen | CD19 | |
| Clone | HIB19 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 510 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Brilliant Violet 510 anti-human CD19 |
About CD19 and Brilliant Violet 510
| CD19 | This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large C-terminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| Brilliant Violet 510 | Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due to its improved signal-to-noise over alternatives like V500 and AmCyan. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation. |
Images
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Human peripheral blood lymphocytes were stained with CD3 APC and CD19 (clone HIB19) Brilliant Violet 510™. |
Citations
| PMID 19155503 | ||
|---|---|---|
| PMID 20015889 | ||
| PMID 20410262 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 510 Excitation and Emission Spectra
$182.00
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