CD25 / PerCP-Cy5.5 / 3C7

Citations: (9)
Product Details
Supplier BioLegend
Catalog #: 101911 (View supplier product page)
Size 25 μg
Price $124.00
Antigen CD25
Clone 3C7
Host Rat
Isotype IgG2b κ
Conjugate PerCP-Cy5.5
Target Species Mouse
Applications FC
Description PerCP/Cyanine5.5 anti-mouse CD25
About CD25 and PerCP-Cy5.5
CD25 The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  CD25 PerCP-Cy5.5 BioLegend antibody anti target:Mouse host:Rat isotype:IgG2b κ clone:3C7

Con A-stimulated C57BL/7 splenocytes (Day 3) stained with 3C7 PerCP/Cy5.5
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PerCP-Cy5.5 Excitation and Emission Spectra