IFN-gamma / PerCP-Cy5.5 / XMG1.2

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Product Details
Supplier BioLegend
Catalog #: 505821 (View supplier product page)
Size 25 μg
Price $118.00
Antigen IFN-gamma
Clone XMG1.2
Host Rat
Isotype IgG1 κ
Conjugate PerCP-Cy5.5
Target Species Mouse
Applications ICS
Description PerCP/Cyanine5.5 anti-mouse IFN-gamma
About IFN-gamma and PerCP-Cy5.5
IFN-gamma This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
Images
  IFN-gamma PerCP-Cy5.5 BioLegend antibody anti target:Mouse host:Rat isotype:IgG1 κ clone:XMG1.2

PMA/Ionomycin-stimlated ( 6hrs ) C57BL/6 splenocytes surface stained with 145-2C11 (CD3) APC and intracellularly stained with XMG1.2 PerCP/Cy5.5

Citations
PerCP-Cy5.5 Excitation and Emission Spectra