CD43 / PerCP-Cy5.5 / 1B11
CD43 / PerCP-Cy5.5 / 1B11
Citations:
(6)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 121223 (View supplier product page) | |
| Size | 25 μg | |
| Price | $153.00 | |
| Antigen | CD43 | |
| Clone | 1B11 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | PerCP-Cy5.5 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | PerCP/Cyanine5.5 anti-mouse CD43 Activation-Associated Glycoform |
About CD43 and PerCP-Cy5.5
| CD43 | This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017] | |
|---|---|---|
| PerCP-Cy5.5 | PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710. |
Images
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Balb/c mouse splenocytes stained with 1B11 PerCP/Cy5.5 |
Citations
| PMID 18354208 | ||
|---|---|---|
| PMID 20943997 | ||
| Additional Sources |
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Reviews & Ratings
PerCP-Cy5.5 Excitation and Emission Spectra
$153.00
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