IFN-gamma / Brilliant Violet 650 / 4S.B3
IFN-gamma / Brilliant Violet 650 / 4S.B3
Citations:
(5)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 502538 (View supplier product page) | |
| Size | 100T | |
| Price | $446.00 | |
| Antigen | IFN-gamma | |
| Clone | 4S.B3 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 650 | |
| Target Species | Human | |
| Applications | ICS | |
| Description | Brilliant Violet 650 anti-human IFN-gamma |
About IFN-gamma and Brilliant Violet 650
| IFN-gamma | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] | |
|---|---|---|
| Brilliant Violet 650 | Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 FITC, and then intracellularly stained with IFN-γ (clone 4S.B3) Brilliant Violet 650™. |
Citations
| PMID 17641010 | ||
|---|---|---|
| PMID 24899193 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 650 Excitation and Emission Spectra
$446.00
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