FluoroFinder

	Supplier	US Biological
	Catalog #:	126713-FITC-100UL (View supplier product page)
	Size	100 μl
	Price	$681.00
	Antigen	FBXW12
	Clone	1A9
	Host	Mouse
	Isotype	IgG2a κ
	Conjugate	FITC
	Target Species	Human
	Applications	ELISA, WB
	Description	FBXW12 (FBW12, FBXO12, FBXO35, F-box/WD Repeat-containing Protein 12, F-box and WD-40 Domain-containing Protein 12, F-box Only Protein 35, MGC120385, MGC120386, MGC120387) (FITC) Mab 1A9

	FBXW12	Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

	Additional Sources

FBXW12 / FITC / 1A9