IgG Secondary Antibody / Alexa Fluor 488 / Poly4054
IgG Secondary Antibody / Alexa Fluor 488 / Poly4054
Citations:
(25)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 405418 (View supplier product page) | |
| Size | 100 μg | |
| Price | $188.00 | |
| Antigen | IgG | |
| Clone | Poly4054 | |
| Host | Goat | |
| Isotype | IgG | |
| Conjugate | Alexa Fluor 488 | |
| Target Species | Rat | |
| Applications | FC, IHC-Fr | |
| Description | Alexa Fluor 488 Goat anti-IgG (minimal x-reactivity) |
About IgG and Alexa Fluor 488
| IgG | This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008] | |
|---|---|---|
| Alexa Fluor 488 | Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A. |
Images
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C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse/human CD45R/B220 (clone RA3-6B2) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 488 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective. |
Citations
| PMID 20702617 | ||
|---|---|---|
| PMID 21212411 | ||
| Additional Sources |
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Reviews & Ratings
Alexa Fluor 488 Excitation and Emission Spectra
$188.00
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