IFN-gamma / PE-Dazzle 594 / XMG1.2

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Product Details
Supplier BioLegend
Catalog #: 505845 (View supplier product page)
Size 25 μg
Price $139.00
Antigen IFN-gamma
Clone XMG1.2
Host Rat
Isotype IgG1 κ
Conjugate PE-Dazzle 594
Target Species Mouse
Applications ICS
Description PE/Dazzle 594 anti-mouse IFN-gamma
About IFN-gamma and PE-Dazzle 594
IFN-gamma This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
PE-Dazzle 594 PE-Dazzle® 594 (RPE-Dazzle 594) from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Dazzle 594. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Dazzle 594, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-Dazzle 594 has an excitation peak at 565 nm and an emission peak at 610 nm, and is suitable alternative to PE-Texas Red, PE-eF610, PE-Alexa Fluor™ 610 or PE-CF®594.
Images
  IFN-gamma PE-Dazzle 594 BioLegend antibody anti target:Mouse host:Rat isotype:IgG1 κ clone:XMG1.2

PMA + Ionomycin-stimulated (6 hours) C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized, and then stained with IFN-γ (clone XMG1.2) PE/Dazzle™ 594 (top) or rat IgG1, κ PE/Dazzle™ 594 isotype control (bottom).

  IFN-gamma PE-Dazzle 594 BioLegend antibody anti target:Mouse host:Rat isotype:IgG1 κ clone:XMG1.2

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PE-Dazzle 594 Excitation and Emission Spectra