CD45 / Brilliant Violet 650 / HI30
CD45 / Brilliant Violet 650 / HI30
Citations:
(16)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 304044 (View supplier product page) | |
| Size | 100T | |
| Price | $401.00 | |
| Antigen | CD45 | |
| Clone | HI30 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Brilliant Violet 650 | |
| Target Species | Human | |
| Applications | FC | |
| Description | Brilliant Violet 650 anti-human CD45 |
About CD45 and Brilliant Violet 650
| CD45 | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] | |
|---|---|---|
| Brilliant Violet 650 | Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad. |
Images
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Human peripheral blood lymphocytes were stained with CD45 (clone HI30) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram). |
Citations
| PMID 18544681 | ||
|---|---|---|
| PMID 21138865 | ||
| PMID 22828869 | ||
| PMID 24648400 | ||
| PMID 25672754 | ||
| PMID 25687281 | ||
| PMID 25687283 | ||
| Additional Sources |
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Reviews & Ratings
Brilliant Violet 650 Excitation and Emission Spectra
$401.00
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