Chk2 / FITC / 2CHK01;same as DCS-293
Chk2 / FITC / 2CHK01;same as DCS-293
Product Details
| Supplier | US Biological | |
|---|---|---|
| Catalog #: | C4201-12X-FITC-100UL (View supplier product page) | |
| Size | 100 μl | |
| Price | $1,204.00 | |
| Antigen | Chk2 | |
| Clone | 2CHK01;same as DCS-293 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | FITC | |
| Target Species | Human | |
| Applications | IF, IP, WB | |
| Description | Chk2 (Checkpoint Kinase 2, Rad 53) (BSA & Azide Free) (FITC) Mab 2CHK01;same as DCS-293 |
About Chk2 and FITC
| Chk2 | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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