ErbB2 / FITC / 3F33, e2 4001
ErbB2 / FITC / 3F33, e2 4001
Product Details
| Supplier | US Biological | |
|---|---|---|
| Catalog #: | C0026-11NX-FITC-100UL (View supplier product page) | |
| Size | 100 μl | |
| Price | $887.00 | |
| Antigen | ErbB2 | |
| Clone | 3F33, e2 4001 | |
| Host | Mouse | |
| Isotype | IgG1 | |
| Conjugate | FITC | |
| Target Species | Human | |
| Applications | IHC, IP, WB | |
| Description | c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free) (FITC) Mab 3F33, e2 4001 |
About ErbB2 and FITC
| ErbB2 | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] | |
|---|---|---|
| FITC | Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark. |
Citations
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