FluoroFinder

	Supplier	US Biological
	Catalog #:	C2433-60C-FITC-100UL (View supplier product page)
	Size	100 μl
	Price	$736.00
	Antigen	CD82
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	FITC
	Target Species	Human
	Applications	IHC, WB
	Description	CD82 (CT) (4F9,C33, GR15, Inducible membrane protein, Inducible membrane protein R2, KAI 1, KAI1, Kangai 1, Kangai1, Metastasis suppressor Kangai 1, R2, R2 leukocyte antigen, SAR2, ST6, Suppression of tumorigenicity 6, Suppression of tumorigenicity 6 prostate, Tetraspanin27, Tspan27) (FITC) Pab

	CD82	This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

	Additional Sources

CD82 / FITC /