FluoroFinder

	Supplier	US Biological
	Catalog #:	032053-FITC-200UL (View supplier product page)
	Size	200 μl
	Price	$711.00
	Antigen	ARHGEF4
	Clone
	Host	Rabbit
	Isotype	IgG
	Conjugate	FITC
	Target Species	Human
	Applications	ELISA, WB
	Description	ARHGEF4, CT (ARHGEF4, KIAA1112, Rho guanine nucleotide exchange factor 4, APC-stimulated guanine nucleotide exchange factor 1) (FITC) Pab

	ARHGEF4	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

	Additional Sources

ARHGEF4 / FITC /