IgM / Alexa Fluor 633 /

Product Details
Supplier Signalway Antibody
Catalog #: L35422-1ML (View supplier product page)
Size 1 ml
Price $505.00
Antigen IgM
Clone
Host Rabbit
Isotype IgG
Conjugate Alexa Fluor 633
Target Species Human
Applications
About IgM and Alexa Fluor 633
IgM Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Alexa Fluor 633 Alexa Fluor™ 633 (AF633, Alexa 633) has an excitation peak at 632nm and an emission peak at 647nm. It can be used with the following analysis platforms: Flow Cytometry, Immunoassay.
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Alexa Fluor 633 Excitation and Emission Spectra