p16INK4a / Alexa Fluor 594 / 4C6/4

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP3-08233AF594 (View supplier product page)
Size 100 ul
Price $519.00
Antigen p16INK4a
Clone 4C6/4
Host Mouse
Isotype IgG2a κ
Conjugate Alexa Fluor 594
Target Species Human, Rat
Applications WB, FC, IHC, ICC/IF, IHC-P
Description p16INK4a/CDKN2A Antibody (4C6/4) [Alexa Fluor 594]
About p16INK4a and Alexa Fluor 594
p16INK4a This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
Alexa Fluor 594 Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.
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Alexa Fluor 594 Excitation and Emission Spectra