RNF43 42694 ALWPWLLMAT (Dextramer) / FITC / 77

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-90325F (View supplier product page)
Size 0.1 ml
Price $549.00
Antigen RNF43 42694 ALWPWLLMAT (Dextramer)
Clone 77
Host Rabbit
Isotype IgG
Conjugate FITC
Target Species Human
Applications ELISA
Description Rabbit RNF43 antibody [FITC] detects Human
About RNF43 42694 ALWPWLLMAT (Dextramer) and FITC
RNF43 42694 ALWPWLLMAT (Dextramer) The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra