Bcl-2 Monoclonal / FITC / Bcl2/100

Citations: (3)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: A15764 (View supplier product page)
Size 100 Tests
Price $233.00
Antigen Bcl-2
Clone Bcl2/100
Host Mouse
Isotype IgG1
Conjugate FITC
Target Species Human
Applications FC
Description Bcl-2 Monoclonal Antibody (Bcl2/100), FITC
About Bcl-2 and FITC
Bcl-2 This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Citations
PMID 24790435
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FITC Excitation and Emission Spectra