CD80 / PerCP-Cy5.5 / 16-10A1

Citations: (2)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: A14895 (View supplier product page)
Size 25 μg
Price $117.00
Antigen CD80
Clone 16-10A1
Host Hamster
Isotype IgG
Conjugate PerCP-Cy5.5
Target Species Mouse
Applications FC
Description CD80 / B7-1 Monoclonal Antibody, PerCP, Cy® 5.5 conjugate (16-10A1). CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.FACS Assay Dependent
About CD80 and PerCP-Cy5.5
CD80 The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
PerCP-Cy5.5 PerCP-Cyanine® 5.5 (PerCP-Cy5.5) is a red-emitting tandem fluorophore that was originally designed to make the PerCP fluorophore more stable and increase signal intensity. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, Cy5.5, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-Cy5.5 has an excitation peak at 482 nm and an emission peak at 695 nm. There are superior alternatives to PerCP and PerCP-Cy5.5 including RealBlue 705 (BD Biosciences), NovaFluor Blue 690 or PerCP-eFluor™ 710.
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PerCP-Cy5.5 Excitation and Emission Spectra