Elongin B / Unconjugated / 10C4
Elongin B / Unconjugated / 10C4
Citations:
(7)
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 629302 (View supplier product page) | |
| Size | 100 μg | |
| Price | $229.00 | |
| Antigen | Elongin B | |
| Clone | 10C4 | |
| Host | Mouse | |
| Isotype | IgG1 κ | |
| Conjugate | Unconjugated | |
| Target Species | Human | |
| Applications | WB, IP | |
| Description | Purified anti-Elongin B |
About Elongin B and Purified
| Elongin B | This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008] |
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Images
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Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder. |
Citations
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Purified Excitation and Emission Spectra
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