CD235a / Alexa Fluor 647 / M2A1

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-81107AF647 (View supplier product page)
Size 0.1 ml
Price $519.00
Antigen CD235a
Clone M2A1
Host Rabbit
Isotype IgG κ
Conjugate Alexa Fluor 647
Target Species Human
Applications ELISA, Dot Blot
Description M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ~ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.
About CD235a and Alexa Fluor 647
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra