CD40 / DyLight 650 / A54 scFv

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-81101C (View supplier product page)
Size 0.1 ml
Price $519.00
Antigen CD40
Clone A54 scFv
Host Rabbit
Isotype IgG λ
Conjugate DyLight 650
Target Species Human
Applications ELISA
Description A54 binds specifically to the D1 domain of CD40/TNFRSF5 (and can only bind to the intact i.e. non-truncated version of CD40/TNFRSF5 and when an AD2 peptide tag has not been added) and can recognise CD40/TNFRSF5 on a B cell line (BJAB). This scFv binds to CD40/TNFRSF5 (immobilized by amine coupling) with a KD within the range of 40nM. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
About CD40 and DyLight 650
CD40 This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
DyLight 650 DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
Citations
Additional
Sources
Reviews & Ratings
Submit a Review

DyLight 650 Excitation and Emission Spectra