F4/80 / Alexa Fluor 532 / CI:A3-1

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-61613AF532 (View supplier product page)
Size 0.1 ml
Price $499.00
Antigen F4/80
Clone CI:A3-1
Host Rat
Isotype IgG1 κ
Conjugate Alexa Fluor 532
Target Species Mouse
Applications FC, IHC
Description This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.
About F4/80 and Alexa Fluor 532
F4/80 This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Alexa Fluor 532 Alexa Fluor™ 532 (AF532, Alexa 532) has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 514 (ATT Bioquest) and iFluor® 546 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 532 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.
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Alexa Fluor 532 Excitation and Emission Spectra