CD45 / APC-Fire 810 / 30-F11

Citations: (2)
Product Details
Supplier BioLegend
Catalog #: 103174 (View supplier product page)
Size 100 μg
Price $382.00
Antigen CD45
Clone 30-F11
Host Rat
Isotype IgG2b κ
Conjugate APC-Fire 810
Target Species Mouse
Applications FC
Description APC/Fire 810 anti-mouse CD45
About CD45 and APC-Fire 810
CD45 The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
APC-Fire 810 APC-Fire™ 810 from BioLegend has an excitation peak at 650nm and an emission peak at 807 nm and is spectrally similar to APC-CF®790. APC-Fire™ 810 is most commonly used in flow cytometry. On a 5-laser Cytek® Aurora, it has very limited spectral spillover into any other channel, so it can be assigned to any antigen with moderate to abundant expression and used in combination with any other fluorophore. This dye is very useful for detecting variably expressed markers, like markers of activation, since it emits so far outside of the autofluorescence range.
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APC-Fire 810 Excitation and Emission Spectra