CD305 / PE / REA447

Citations: (3)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-126-118 (View supplier product page)
Size 30 tests in 60 µL
Price $120.00
Antigen CD305
Clone REA447
Host Human
Isotype IgG1
Conjugate PE
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IF, IHC, FC
Description Clone REA447 recognizes the human CD305 antigen, a single-pass type I membrane protein which is also known as leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1). CD305 contains a single extracellular immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. It is structurally related to several other inhibitory Ig superfamily members. CD305 is expressed on almost all immune cells, including NK cells, T cells, B cells, monocytes, monocyte-derived dendritic cells, eosinophils, basophils, and mast cells. Besides being expressed on differentiated peripheral blood cells, the receptor is also present on CD34+ hematopoietic progenitor cells and on the majority of thymocytes. Cross-linking of CD305 on human NK cells delivers a potent inhibitory signal that is capable of inhibiting target cell lysis by resting and activated NK cells. Furthermore, CD305 can inhibit the cytotoxic activity of effector T cells upon CD3 cross-linking or antigen stimulation. In primary B cells, CD305 cross-linking leads to decreased BCR-induced calcium mobilization and down-regulation of Ig and cytokine production. | Additional information: Clone REA447 displays negligible binding to Fc receptors.
About CD305 and PE
CD305 The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
PMID 9285412
PMID 18063695
PMID 24415628
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PE Excitation and Emission Spectra