Perforin / PE / REAL787

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-125-274 (View supplier product page)
Size 200 μl
Price $505.00
Antigen Perforin
Clone REAL787
Host cell line
Isotype IgG
Conjugate PE
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), IHC, IF
Description Clone REAL787 is an antibody fragment derived from the full Perforin antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. | Clone REAL787 recognizes the human perforin, a 70 kD cytolytic protein, which is expressed by cytotoxic T lymphocytes and natural killer (NK) cells, and stored in cytoplasmic granules. Upon cell activation perforin is released and functions as one of the major effector molecule to mediate killing of target cells. Perforin-mediated cytotoxicity plays an essential role in various autoimmune diseases. | For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
About Perforin and PE
Perforin This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
PMID 10196391
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PE Excitation and Emission Spectra